Studies on the Biological and Antigenic Properties of ESP-1 Type C Virus Particles1
نویسندگان
چکیده
ESP-1 virus provides a helper function to defective Friend spleen focus-forming virus (SFFV). This helper activity is demonstrable only in B-type mice (Fv-lbb) under conditions in which the N-tropic lymphatic leukemia-inducing virus contained in the Friend virus complex is inhibited. ESP-1 virus therefore expresses a B-tropic host range in vivo with respect to its ability to provide a helper function to defective SFFV. Further, this helper activity is directly associated with the presence of the ESP-1 virus since activity was recovered only from gradient samples ranging from 1.15 to 1.17 g/ml. All samples contained ESP-1 virus and Mycoplasma. However, Mycoplasma derived from the ESP-1 cell line and maintained in broth culture only enhanced SFFV titer estimates and failed to provide a helper function to SFFV. ESP-1 virus helper activity is dose dependent and heat labile and is neutralized by rat antiserum directed against both whole and Tween:etherdisrupted ESP-1 virus. Further, ESP-1 virus is not neutralized by type-specific murine antiserum directed against both the Rauscher virus complex and the lymphatic leukemia-inducing helper virus component of the Rauscher virus complex and Moloney leukemia virus. These findings, together with the observations that these same murine antisera neutralized human-embryo-kidney-cell-grown Rauscher leukemia virus, suggest that the type C virus designated ESP-1 has some of the biological properties of the murine leukemia viruses (i.e., helper activity for the induction of leukemia in mice) but does not contain type-specific envelope antigen(s) characteristic of Moloney leukemia virus, N-tropic Friend lymphatic leukemiainducing virus, or human-embryo-kidney-cell-grown Rauscher leukemia virus.
منابع مشابه
Ferritin-labeled Antibody Studies of Feline C-type Particles1
The application of an indirect ferritin-labeled antibody method to tissue culture lines established from cats suffering from various malignant diseases revealed tagging of viruses and of certain areas of the cell surface membranes. Dog antifeline sarcoma and cat antifeline sarcoma sera were used as the intermediate sera on feline C-type virus-positive lines established from 3 cases of malignant...
متن کاملGenetic and antigenic analysis of type O and A FMD viruses isolated in Iran
FMD is one of the most highly contagious diseases of animals, caused by RNA virus belong to Picornaviridae family and Aphtovirus genus. A broad host range and occurrence of FMDV as seven serotypes and also intratypic antigenic variation without clear cut demarcations, which interferes with a concept of sub typing these factors make difficult conditions to diagnosis, control and eradication of...
متن کاملStudies in Borreliae II. Some Immunologic, Biochemical and Physical Properties of the Antigenic Components of Borrelia turicatae
متن کامل
The Effect of Short-Range Radiation of Type C and B Ultraviolet on the Mechanical Properties of Skin Fibroblasts
The effect of UV beam, which has been emitted from a natural or a manmade source on cells has been studied in previous studies for several times. Radiation of this beam can have different effects on DNA of the cell, cytotoxicity, the structure of cellular proteins and their mechanical properties based on radiation period or frequency. The effect of radiation of two types of beams, namely UVB an...
متن کاملThe Relationship between Hepatitis C Genotypes and Viral Load: a study on hepatitis C patients in Kerman, Iran using Real-time PCR
Abstract Background: Distribution of hepatitis C virus genotypes varies geographically and can be associated with clinical aspects. The virus has six major genotypes and eleven sub-genotypes. According to the recent studies, in hepatitis C patients, treatment duration and ribavirin dosage should be determined based on the type of virus genotype. Be...
متن کامل